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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Exonuclease III ： ウィキペディア英語版 Exonuclease III Exonuclease III (ExoIII) is an enzyme that belongs to the exonuclease family. ExoIII catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of duplex DNA.〔 A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules.〔 ==Function== The preferred substrates are blunt or recessed 3´-termini, although ExoIII also acts at nicks in duplex DNA to produce single-strand gaps. The enzyme is not active on single-stranded DNA, and thus 3´-protruding termini are resistant to cleavage. The degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This property is used to produce unidirectional deletions from a linear molecule with one resistant (3´-overhang) and one susceptible (blunt or 5´-overhang) terminus. ExoIII activity depends partially on the DNA helical structure and displays sequence dependence (C>A=T>G). ExoIII has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Exonuclease III」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.